And Genomes (KEGG) database as our supply of annotations, 583 out of 803 Populus genes annotated as being involved inFigure five Gene ontology (GO) annotation of saltresponsive genes compared in between P. euphratica and P. pruinosa. WEGO was utilised to make the graph. We divided the sets in to the three key GO domains: biological course of action, cellular component and molecular function, along with the quantity (suitable yaxis) and percentage (left yaxis) of genes had been calculated.Zhang et al. BMC Genomics 2014, 15:337 http://www.biomedcentral.com/14712164/15/Page six ofTable two Gene ontology (GO) enrichment analyses for saltresponsive genes compared amongst P. euphratica and P. pruinosaGO term GO:0006950 GO:0050896 GO:0006952 GO:0009607 GO:0009308 GO:0044264 GO:0044042 GO:0006073 GO:0044260 GO:0048037 GO:0050662 GO:0030414 GO:0004866 GO:0050660 GO:0016491 GO:0004857 GO:0030234 GO:0030246 GO:0004497 GO:0003824 GO:0046906 GO:0020037 GO:0017171 GO:0008236 GO:0016758 GO:0016762 GO:0048046 GO:0044464 GO:0005623 GO:0005576 GO:0030312 GO:Onto2 P P P P P P P P P F F F F F F F F F F F F F F F F F C C C C C CDescription Response to tension Response to stimulus Defense response Response to biotic stimulus Amine metabolic process Cellular polysaccharide metabolic process Glucan metabolic procedure Cellular glucan metabolic course of action Cellular macromolecule metabolic procedure Cofactor binding Coenzyme binding Peptidase inhibitor activity Endopeptidase inhibitor activity FAD binding Oxidoreductase activity Enzyme inhibitor activity Enzyme regulator activity Carbohydrate binding Monooxygenase activity Catalytic activity Tetrapyrrole binding Heme binding Serine hydrolase activity Serinetype peptidase activity Transferase activity, transferring hexosyl groups Xyloglucan:xyloglucosyl transferase activity Apoplast Cell part Cell Extracellular region External encapsulating structure Cell wallNumber Zscore 50 54 16 15 22 13 13 13 159 67 53 21 21 33 191 32 33 24 42 504 64 64 13 13 16 ten 10 169 169 12 18 18 7.98386-83-5 web 2 six.7 six.4 5.9 3.5 3.6 three.six 3.6 3.six eight.1 7.9 7.9 7.9 7.4 six.6 six.5 six.2 four.five four three.7 3.5 three.five 3.four three.four three.1 three.1 three.1 3.two three.2 two.four 2.eight 2.P. euphratica Mean3 five.1 four.6 7.7 7.4 3.7 4.three four.3 four.three 1 four.9 5.4 eight.three 8.three 6.3 2.five five.7 five.4 4.6 three.2 1 two.three 2.three 4.1 4.1 three.3 four.two 4.2 0.87 0.87 three two.eight two.8 FDR four.50e11 1.60e09 1.50e08 4.20e07 0.047 0.036 0.036 0.036 0.035 three.90e14 two.00e13 2.50e13 two.50e13 1.40e11 two.2-(3-Butyn-1-yloxy)acetic acid web 60e09 six.PMID:24624203 50e09 three.80e08 0.00052 0.0052 0.019 0.042 0.042 0.059 0.059 0.2 0.2 0.047 0.03 0.03 0.33 0.11 0.11 Zscore 3.1 three.1 three.2 three.four 2.three four.6 4.6 4.six 2.two three.8 three.5 four.3 four.3 2.9 2.3 three.4 three.2 two.3 1.1 0.93 0.12 0.12 3.five 3.5 3.8 four.two four.two 1.1 1.1 three.3 3.eight three.P. pruinosa Mean 1.8 1.8 two.9 three.1 2 3 3 3 0.11 1.9 2 three.2 three.two 2 1.1 two.three 2.1 1.9 1.1 0.72 0.65 0.65 two.1 2.1 two.1 3.1 3.1 0.36 0.36 two.1 1.9 1.9 FDR 0.21 0.21 0.11 0.063 1 0.00034 0.00034 0.00034 1 0.011 0.034 0.0015 0.0015 0.37 1 0.066 0.13 1 1 1 1 1 0.036 0.036 0.011 0.0023 0.00054 1 1 0.02 0.0033 0.The 1430 DEGs have been analyzed by parametric analysis of gene set enrichment (Page). 2 Onto, Gene Ontology domain; P, biological course of action; F, molecular function; C, cellular element. three Imply, imply log2 expression ratio, 0 represents upregulation, 0 represents downregulation.hormone biosynthesis [52] have been detected inside the four libraries and 59 of those genes have been differentially expressed in either P. euphratica or P. pruinosa below salt pressure (More file 3). Amongst these hormone biosynthesisrelated DEGs, 37 were identified in P. euphratica, of which 36 were upregulated and one was downregul.
